來源：深圳醫(yī)學(xué)科學(xué)院

個人簡介

范瀟博士于 2014 年獲清華大學(xué)學(xué)士學(xué)位，2019 年獲清華大學(xué)博士學(xué)位。2019 年至 2025 年間，先后于普林斯頓大學(xué)及洛克菲勒大學(xué)/霍華德 · 休斯醫(yī)學(xué)研究所（HHMI）從事博士后研究。2025 年 8 月全職加入深圳醫(yī)學(xué)科學(xué)院，任生物構(gòu)造與互作研究所特聘研究員。

范瀟博士長期致力于融合多學(xué)科前沿技術(shù)方法，以推動冷凍電鏡技術(shù)創(chuàng)新，并聚焦于重大疾病相關(guān)膜蛋白-藥物分子相互作用機制的結(jié)構(gòu)生物學(xué)研究。通過自主研發(fā)的冷凍電鏡全流程創(chuàng)新技術(shù)體系，克服了該領(lǐng)域長期存在的技術(shù)瓶頸，成功實現(xiàn)了超小分子量蛋白及近生理狀態(tài)下膜蛋白的高分辨率結(jié)構(gòu)解析，并揭示了電壓門控鈉離子/鈣離子通道的結(jié)構(gòu)與藥理作用機制等一系列重要發(fā)現(xiàn)。

主要獎項與榮譽

人類前沿科學(xué)計劃 (HFSP) 博士后基金獎

課題組研究方向

范瀟課題組將立足于多學(xué)科交叉領(lǐng)域，發(fā)揮技術(shù)優(yōu)勢，開發(fā)新型冷凍電鏡成像技術(shù)及高效樣品制備與數(shù)據(jù)處理方法，構(gòu)建針對天然膜體系的膜蛋白原位結(jié)構(gòu)與功能解析平臺。并將依托該平臺開展復(fù)雜生物膜環(huán)境的高效原位研究，通過多維度解析關(guān)鍵大分子的空間構(gòu)型、相互作用網(wǎng)絡(luò)及跨尺度組織模式，深入探索生物膜系統(tǒng)間的耦合與調(diào)控機制，為相關(guān)疾病干預(yù)提供理論基礎(chǔ)。

主要論文集

* Equal contributions # Corresponding author

主要論文集：

1.Xiao Fan*, Jiaofeng Chen*, Xiaoshuang Huang, Zhanfeng Hou, Yuzhen Xie, Zigang Li, Nieng Yan#, and Jian Huang# (2025). Phrixotoxin-3 inhibits human Nav1.6 through tri-pocket binding. Cell Research, 35(8):610-613.

2.Zi Yang*, Xiao Fan*, and Hongwei Wang# (2025). Near-atomic resolution single-particle 3D reconstruction using a single Cryo-EM micrograph. Journal of Chinese Electron Microscopy Society, 44 (1): 1000-6281

3.Jian Huang*, Xiao Fan*, Xueqin Jin, Chen Lyu, Qinmeng Guo, Tao Liu, Jiaofeng Chen, Ama?l Davakan, Philippe Lory, and Nieng Yan# (2024). Structural basis for human Cav3.2 inhibition by selective antagonists. Cell Research, 0:1–11.

4.Yang, Zi, Jingjin Fan, Jia Wang, Xiao Fan, Zheng Ouyang, Hong-Wei Wang#, and Xiaoyu Zhou# (2024). Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects. Nature Methods: 1-10.

5.Kristina Solorio-Kirpichyan, Xiao Fan*, Dmitrij Golovenko*, Andrei A. Korostelev, Nieng Yan, and Alexei Korennykh# (2024). Cryo-EM structure of histidyl-tRNA synthetase-like domain reveals activating crossed helices at the core of GCN2. PNAS nexus 3, no. 12: pgae528.

6.Xiao Fan*#, Jian Huang*, Xueqin Jin and Nieng Yan# (2023). Cryo-EM structure of human voltage-gated sodium channel Nav1.6. Proceedings of the National Academy of Sciences, 120(5), e2220578120.

7.Jian Huang*, Xiao Fan*#, Xueqin Jin, Liming Teng and Nieng Yan#. (2023). Dual-pocket inhibition of Nav channels by the antiepileptic drug lamotrigine. Proceedings of the National Academy of Sciences, 120(41), e2309773120.

8.Qiurong Wu*, Jian Huang*#, Xiao Fan*#, Gaoxingyu Huang, Xueqin Jin, Xiaojing Pan# and Nieng Yan# (2023). Structural mapping of Nav1.7 antagonists. Nature Communications, 14 (1), 3224.

9.Jian Huang*, Xiao Fan*, Xueqin Jin, Sooyeon Jo, Hanxiong Bear Zhang, Akie Fujita, Bruce P. Bean#, and Nieng Yan# (2023). Cannabidiol inhibits Nav channels through two distinct binding sites. Nature Communications, 14(1), 3613.

10.Shuai Gao, Xia Yao, Jiaofeng Chen, Gaoxingyu Huang, Xiao Fan, Lingfeng Xue, Zhangqiang Li, Tong Wu, Yupeng Zheng, Jian Huang, Xueqin Jin, Yan Wang, Zhifei Wang, Yong Yu, Lei Liu, Xiaojing Pan, Chen Song and Nieng Yan. (2023). Structural basis for human Cav1.2 inhibition by multiple drugs and the neurotoxin calciseptine. Cell, 186(24), 5363-5374.

11.Peng Huang, Raminta Venskutonyt?, Rashmi B. Prasad, Hamidreza Ardalani, Sofia W. de Maré, Xiao Fan, Ping Li, Peter Spégel, Nieng Yan, Pontus Gourdon, Isabella Artner and Karin Lindkvist-Petersson (2023). Cryo-EM structure supports a role of AQP7 as a junction protein. Nature Communications, 14(1), 600.

12.Xia Yao, Yan Wang, Zhifei Wang, Xiao Fan, Di Wu, Jian Huang, Alexander Mueller, Sarah Gao, Miaohui Hu, Carol V. Robinson, Yong Yu, Shuai Gao, and Nieng Yan (2022). Structures of the R-type human Cav2.3 channel reveal conformational crosstalk of the intracellular segments. Nature communications, 13(1): 7358.

13.Xia Yao, Shuai Gao, Jixin Wang, Zhangqiang Li, Jian Huang, Yan Wang, Zhifei Wang, Jiaofeng Chen, Xiao Fan, Weipeng Wang, Xueqin Jin, Xiaojing Pan, Yong Yu, Armando Lagrutta, Nieng Yan (2022). Structural basis for the severe adverse interaction of sofosbuvir and amiodarone on L-type Cav channels. Cell, 185(25), 4801-4810.

14.Jiemin Shen*, Miaohui Hu*, Xiao Fan*#, Zhenning Ren, Corinne Portioli, Xiuwen Yan, Mingqiang Rong, Ming Zhou# (2022). Extracellular domain of PepT1 interacts with TM1 to facilitate substrate transport. Structure, 7(30): 1035-1041.e3.

15.Heng Ni*, Xiao Fan*, Feng Zhou, Galio Guo, Jae Young Lee, Nadrian C Seeman, Do-Nyun Kim, Nan Yao, Paul M Chaikin, and Yimo Han# (2022). Direct visualization of floppy two-dimensional DNA origami using cryogenic electron microscopy. iScience, 6(25): 104373.

16.Xiao Fan (2022). "Breaking down the barriers in cryo-EM analysis of membrane proteins." Foundations of Crystallography, 2022, 78: A240-A240.

17.Xia Yao*, Xiao Fan*#, Nieng Yan# (2020). Cryo-EM analysis of a membrane protein embedded in the liposome. Proceedings of the National Academy of Sciences, 117(31): 18497-18503.

18.Yimo Han*, Xiao Fan*, Haozhe Wang, Fang Zhao, Christopher Tully, Jing Kong, Nan Yao and Nieng Yan# (2020). High-yield monolayer graphene grids for near-atomic resolution cryoelectron microscopy. Proceedings of the National Academy of Sciences, 117, 1009-1014.

19.Yaping Pan, Zhenning Ren, Shuai Gao, Jiemin Shen, Lie Wang, Zhichun Xu, Ye Yu, Preetham Bachina, Hanzhi Zhang, Xiao Fan, Arthur Laganowsky, Nieng Yan & Ming Zhou (2020). Structural basis of ion transport and inhibition in ferroportin. Nature communications 11(1), 1-11.

20.Xiao Fan*, Jia Wang*, Xing Zhang, Zi Yang, Jin-Can Zhang, Lingyun Zhao, Hai-Lin Peng, Jianlin Lei#, and Hong-Wei Wang# (2019). Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nature communications 10, 2386.

21.Hong-Wei Wang and Xiao Fan (2019). Challenges and opportunities in cryo-EM with phase plate. Current Opinion in Structural Biology, 58, 175–182.

22.Xiao Fan*, Lingyun Zhao*, Chuan Liu, Jin-Can Zhang, Kelong Fan, Xiyun Yan, Hai-Lin Peng, Jianlin Lei# and Hong-Wei Wang# (2017). Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM. Structure 25, 1623-1630.

我們長期為科研用戶提供前沿資訊、實驗方法、選品推薦等服務(wù)，并且組建了 70 多個不同領(lǐng)域的專業(yè)交流群，覆蓋PCR、細(xì)胞實驗、蛋白研究、神經(jīng)科學(xué)、腫瘤免疫、基因編輯、外泌體、類器官等領(lǐng)域，定期分享實驗干貨、文獻解讀等活動。

添加實驗菌企微，回復(fù)【】中的序號，即可領(lǐng)取對應(yīng)的資料包哦～

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